Benjamin Maxwell's daughter, Sofia, read Charlotte's Web when she was about five years old. Decades later, in January 2386 when Maxwell was the janitor aboard the Robert Hooke Research Station, the arachnoform Honey was taken over by Anatoly Finch's experiment Mother. Honey/Mother communicated with Maxwell by referencing the phrase "some bug" (instead of "some pig") from the book. She did this by writing the words in her silk on the deck, and also showed him an image of the front cover of the book on a viewscreen. (DS9 novel: Force and Motion)
In August 2382, Benjamin Sisko gave his daughter Rebecca a hardcover book, with an illustrated cover which depicted a red-haired girl and some farm animals watching a spider spin a web. (ST - Typhon Pact novel: Plagues of Night)
- While not explicitly named as Charlotte's Web, the book Sisko gave Rebecca is implied to be the titular novel.