Nautilus could refer to:
- Lifeforms
- Nautilus (mollusk), a type of sea mollusk
- Seagoing craft
- Nautilus (submarine), a submersible used on Timshel in 2267
- Nautilus (submersible), the first ship to explore Europa's ocean
- Nautilus (Jules Verne), the fictional submarine of Captain Nemo
- USS Nautilus (submarine), first atomic-powered submarine on Earth
- Starships and shuttles
- Nautilus (amphishuttle), an amphishuttle used on Pacifica in 2310
- Nautilus class, a type of Federation starship
- USS Nautilus (NCC-31910), a Miranda-class ship
- USS Nautilus (Akira-class)
- USS Nautilus (Kelvin timeline), a Kelvin timeline starship
- USS Nautilus (Steamrunner-class)
- Nautilus (Constitution-class)/ Nautilus (26th century), a starship from the 26th century
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