Shuttle 01 was a Federation Starfleet type-18 shuttlepod assigned to the starship USS Defiant in the 2370s. (DS9 reference: Star Trek: Deep Space Nine Technical Manual)
Early in the year 2371, Odo and Kira Nerys escaped from a Jem'Hadar attack on the Defiant in Shuttle 01. (DS9 episode: "The Search")
Later that year, Benjamin Sisko and Kira used Shuttle 01 to generate a subspace field around comet fragments travelling through the Bajoran wormhole to prevent silithium from the comet reacting with the wormhole. (DS9 episode: "Destiny")
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