Guardian could refer to:
- Starships and vehicles
- USS Guardian (NCC-26244), a Federation Miranda-class starship active in the late 23rd century
- Guardian-class (frigate), a class of frigate used by Starfleet in the mid-23rd century of the alternate reality created by Nero's temporal incursion
- Guardian-class (cruiser), a class of cruiser used by Starfleet in the early 25th century
- USS Guardian (NCC-47501), lead ship of the Guardian-class
- TDS Guardian - a Trill starship
- Guardian (Mirak) - a Mirak starship
- Titles and persons
- Trill Guardian - a caretaker of Trill symbionts
- Guardian (Betan) - a prophesied protector of Landru
- Guardian (Dreman) - the head of government on Drema IV
- Guardian (Mercan) - Guardians on the planet Mercan
- Guardian (Valzhan) - a keeper of Valzhan clan histories
- Relic Guardian - a protector in the Denar system
- Other
- Guardian caste - a division of Orishan society
- Guardian of Forever - a time-space portal
- Operation Guardian - a military engagement in defense of the Guardian of Forever
- Stories
- "Guardians" - a short story
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